COMPOSITION OF THE IMPREGNATING SOLUTION FOR BACTERIAL BIOMINERALIZATION AND FEATURES OF ITS INTERACTION WITH CEMENT SYSTEM COMPONENTS
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Abstract
Building materials and products are subjected to various types of corrosion, the development of which is associated with a large number of variable factors occurring in a complex relationship. The speed and nature of corrosion processes depends on the permeability of concrete products. The migration of aggressive substances in the body of the concrete matrix as a result of transport mechanisms (filtration, capillary transfer, diffusion) leads to a change in porosity and, as a consequence, the physical and mechanical properties of materials. The intensity of transport processes is determined on the one hand by internal parameters (the nature and composition of the binder and other components of the cement and concrete system, the size of aggregates and pores), on the other – by external parameters (the condition and degree of uniformity of the surface of the product, the presence of protective coatings, impregnations, insulation, etc.). In turn, it was found that microbial-induced calcite deposition contributes to an increase and/or prolongation of the resistance of materials to external aggressive factors.
In order to optimize the process of applying an impregnating biocementitious composition, a hypothesis has been proposed about the possibility of introducing bacterial inoculate and precursors into the composition of the emulsion. The use of a polysiloxane emulsion based on a water-soluble polymer intended for the hydrophobization of the surface of cement concrete products, due to its biopositivity due to the absence of volatile organic substances in its composition as a solvent of hydrophobizing resin, is proposed as a base composition. To establish the influence of individual components and technological features of emulsion production on the producing ability of bacteria, an assessment of the effect of organosilicon liquid GKZH-94, polyvinyl alcohol (PVA), temperature and intensity of dispersion (emulsification) on them was carried out.
Subject: Influence of polysiloxane emulsion components on the bacterial activity of B. pumilus.
Materials and methods: Substantiation of the ways of possible technological solutions and their implementation in the selection of the composition of the impregnating solution used for bacterial mineralization in the cement system together with the components of the polysiloxane emulsion.
Results: The absence of harmful effects of both PVA and GKZH on B. pumilus bacteria was recorded. Heating the PVA solution with a bacterial inoculum to 60° C led to sporulation of more than 80% of bacteria. It has been established that high-speed dispersion causes cell damage, there is a sporulation of bacteria up to 1,3×106 CFU/ml against the initial value of 2×108 CFU/ml.
Conclusions: The analysis of the influence of individual components and technological operations of obtaining an emulsion on the preservation of the viability of bacterial cells confirmed the prospects of the expressed hypothesis.
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References
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